BME Seminar Series: James Reinhardt, PhD Candidate of Biomedical Engineering, OSU

James will present "Simulating Collagen Network Remodeling Using An Agent-Based Model"

Simulating Collagen Network Remodeling Using An Agent-Based Model

James W. Reinhardt, Gooch Lab

Department of Biomedical Engineering, The Ohio State University

In vivo, cells continuously maintain their local ECM through a delicate balance of matrix synthesis, chemical modification, degradation, and the application of traction forces. It is believed that a breakdown of this balance and subsequent changes to the mechanical properties of the ECM could result in the loss of homeostasis and promote pathogenesis such as cancer metastasis and fibrosis. Therefore, a greater understanding of how cells locally remodel the ECM and how the microstructural properties of the ECM influence this remodeling has implications for the treatment and prevention of many different diseases. Inspired by in vitro experiments using reconstituted type I collagen hydrogels, I have developed a novel agent-based model of cell-mediated collagen network remodeling in NetLogo. I have used this model to explore how collagen concentration influences the mechanical properties of cell-free collagen networks subjected to tensile or shear strain. I have also quantified cell traction induced remodeling around single, isolated cells and between pairs of cells. In addition, I have shown that the simple way I model cell traction force is sufficient for a cells to exhibit directional movement on a network possessing a stiffness gradient (i.e. durotaxis).

                       This work was supported by NSF CMMI-1334757.