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Daniel Gallego Perez

  • Assistant Professor, General Surgery
  • Assistant Professor, Biomedical Engineering
  • 473 W 12th Ave, Room 501
    Columbus, OH 43210

About

Education

  • B.S., Antioquia School of Engineering, 2003
  • Ph.D., The Ohio State University, 2011

 

Key Honors and Distinctions

  • Lumley Interdisciplinary Research Award, 2017.
  • William G. Lowrie Department of Chemical and Biomolecular Engineering, Outstanding Post-Doc Award for Research Excellence, 2014.
  • CWRU Society for Biomaterials – Biomaterials Day, Student Presentation Award, 2010.
  • Ohio State Biomedical Engineering Conference, Best Presentation in Micro/Nanotechnology, 2009.
  • Society for Biomaterials Meeting, Student Travel Achievement Recognition, 2009.
  • Biomedical Engineering Society Meeting, Student Travel Award, 2008.
  • Ohio State Biomedical Engineering Conference, Best Presentation in Micro/Nanotechnology, 2008.
  • The 8th New Jersey Symposium on Biomaterials Science, Student Travel Scholarship, 2006.

 

RESEARCH AREAS – Gallego-Perez NanoMedicine Lab https://gallegopereznanomed.engineering.osu.edu/

  • Lab-on-a-Chip platforms, single cell analyses, gene delivery, cell reprogramming
  • Graduate student research opportunities are currently available. [indicate whether you have grad student or postdoc opportunities in the lab][D1] Open positions (https://gallegopereznanomed.engineering.osu.edu/)

 

  • PUBLICATIONS

https://scholar.google.com/citations?user=aXJcCFgAAAAJ&hl=en

 

  • PATENTS
    • Hansford D, Schley J, Gallego-Perez D. “Electroosmotic devices for fluid handling” US Patent #61/883,592. 9/27/13.
    • Pal D, Gallego-Perez D, Ghatak S, Lee LJ, Sen CK. “compositions and methods for reprogramming somatic cells into induced endothelial cells”. Provisional patent filed (2016).

The Nano Medicine Laboratory at OSU is dedicated to the development and implementation of novel micro-/nanoscale platforms (e.g., Lab-on-Chip devices, gene delivery chips, tissue engineering scaffolds) for applications in fundamental and/or translational medical research.

Chapters

2015

  • Wu, Y, Gallego-Perez, D, Lee, LJ. 2015. "Microwell Array-Mediated Delivery of Lipoplexes Containing Nucleic Acids for Enhanced Therapeutic Efficacy." In RNA interference: challenges and therapeutic opportunities, edited by Sioud, M, New York: Springer New York.

2012

  • Pelaez-Vargas, A, Gallego-Perez, D, Gomez, D, Fernandes, MH, Hansford, D, Monteiro, FJ. 2012. "Propagation of Human Bone Marrow Stem Cells for Craniofacial Applications." In Stem Cells and Cancer Stem Cells: Therapeutic Applications in Disease and Injury, New York: Springer New York.
  • Pelaez-Vargas, A, Gallego-Perez, D, Higuita-Castro, N, Carvalho, A, Grenho, L, Arismendi, JA, Fernandes, MH, Ferraz, MP, Hansford, DJ, Monteiro, FJ. 2012. "Micropatterned Coatings for Guided Tissue Regeneration in Dental Implantology." In Cell Interaction, Rijeka: InTech.

Journal Articles

2016

  • Chang,Lingqian; Bertani,Paul; Gallego-Perez,Daniel; Yang,Zhaogang; Chen,Feng; Chiang,Chiling; Malkoc,Veysi; Kuang,Tairong; Gao,Keliang; Lee,L,James; Lu,Wu, 2016, "3D nanochannel electroporation for high-throughput cell transfection with high uniformity and dosage control.." Nanoscale 8, no. 1, 243 - 252.
  • Gallego-Perez,Daniel; Otero,Jose,J; Czeisler,Catherine; Ma,Junyu; Ortiz,Cristina; Gygli,Patrick; Catacutan,Fay,Patsy; Gokozan,Hamza,Numan; Cowgill,Aaron; Sherwood,Thomas; Ghatak,Subhadip; Malkoc,Veysi; Zhao,Xi; Liao,Wei-Ching; Gnyawali,Surya; Wang,Xinmei; Adler,Andrew,F; Leong,Kam; Wulff,Brian; Wilgus,Traci,A; Askwith,Candice; Khanna,Savita; Rink,Cameron; Sen,Chandan,K; Lee,L,James, 2016, "Deterministic transfection drives efficient nonviral reprogramming and uncovers reprogramming barriers.." Nanomedicine : nanotechnology, biology, and medicine 12, no. 2, 399 - 409.
  • Chang,Lingqian; Bertani,Paul; Gallego-Perez,Daniel; Yang,Zhaogang; Chen,Feng; Chiang,Chiling; Malkoc,Veysi; Kuang,Tairong; Gao,Keliang; Lee,L,James; Lu,Wu, 2016, "3D nanochannel electroporation for high-throughput cell transfection with high uniformity and dosage control." NANOSCALE 8, no. 1, 243 - 252.
  • Kim,Sung-Hak; Ezhilarasan,Ravesanker; Phillips,Emma; Gallego-Perez,Daniel; Sparks,Amanda; Taylor,David; Ladner,Katherine; Furuta,Takuya; Sabit,Hemragul; Chhipa,Rishi; Cho,Ju,Hwan; Mohyeldin,Ahmed; Beck,Samuel; Kurozumi,Kazuhiko; Kuroiwa,Toshihiko; Iwata,Ryoichi; Asai,Akio; Kim,Jonghwan; Sulman,Erik,P; Cheng,Shi-Yuan; Lee,L,James; Nakada,Mitsutoshi; Guttridge,Denis; Dasgupta,Biplab; Goidts,Violaine; Bhat,Krishna,P; Nakano,Ichiro, 2016, "Serine/Threonine Kinase MLK4 Determines Mesenchymal Identity in Glioma Stem Cells in an NF-kappa B-dependent Manner." CANCER CELL 29, no. 2, 201 - 213.

2015

  • Zhao,Xi; Huang,Xiaomeng; Wang,Xinmei; Wu,Yun; Eisfeld,Ann-Kathrin; Schwind,Sebastian; Gallego-Perez,Daniel; Boukany,Pouyan,E; Marcucci,Guido,I; Lee,Ly,James, 2015, "Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia." ADVANCED SCIENCE 2, no. 12, 1500111 -
  • Bertani,Paul; Lu,Wu; Chang,Lingqian; Gallego-Perez,Daniel; Lee,Ly,James; Chiang,Chiling; Muthusamy,Natarajan, 2015, "Bosch etching for the creation of a 3D nanoelectroporation system for high throughput gene delivery." JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B 33, no. 6, 06F903 -
  • Malkoc,Veysi; Gallego-Perez,Daniel; Nelson,Tyler; Lannutti,John,J; Hansford,Derek,J, 2015, "Controlled neuronal cell patterning and guided neurite growth on micropatterned nanofiber platforms." JOURNAL OF MICROMECHANICS AND MICROENGINEERING 25, no. 12, 125001 -
  • Holfinger,Steven,J; Reinhardt,James,W; Reen,Rashmeet; Schultz,Kevin,M; Passino,Kevin,M; Ackerman,William,E; Kniss,Douglas,A; Sander,Leonard,M; Gallego-Perez,Daniel; Gooch,Keith,J, 2015, "Pancreatic Epithelial Cells Form Islet-Like Clusters in the Absence of Directed Migration." CELLULAR AND MOLECULAR BIOENGINEERING 8, no. 3, 496 - 506.
  • Wu,Yun; Gallego-Perez,Daniel; Lee,L,James, 2015, "Microwell array-mediated delivery of lipoplexes containing nucleic acids for enhanced therapeutic efficacy.." Methods in molecular biology (Clifton, N.J.) 1218, 131 - 142.
  • Zhao,Xi; Wu,Yun; Gallego-Perez,Daniel; Kwak,Kwang,Joo; Gupta,Cherry; Ouyang,Xilian; Lee,L,James, 2015, "Effect of Nonendocytic Uptake of Nanoparticles on Human Bronchial Epithelial Cells." ANALYTICAL CHEMISTRY 87, no. 6, 3208 - 3215.
  • Czeisler,Catherine; Gallego-Perez,Daniel; Ortiz,Cristina; Gygli,Patrick; Askwith,Candice; Lee,L,James; Otero,Jose, 2015, "Nuclear Reprogramming with Hindbrain Patterning Genes by Nanochannel Electroporation." FASEB JOURNAL 29, 1018.4 -
  • Chang,Lingqian; Howdyshell,Marci; Liao,Wei-Ching; Chiang,Chi-Ling; Gallego-Perez,Daniel; Yang,Zhaogang; Lu,Wu; Byrd,John,C; Muthusamy,Natarajan; Lee,L,James; Sooryakumar,Ratnasingham, 2015, "Magnetic Tweezers-Based 3D Microchannel Electroporation for High-Throughput Gene Transfection in Living Cells." SMALL 11, no. 15, 1818 - 1828.
  • Chang,Lingqian; Gallego-Perez,Daniel; Zhao,Xi; Bertani,Paul; Yang,Zhaogang; Chiang,Chi-Ling; Malkoc,Veysi; Shi,Junfeng; Sen,Chandan,K; Odonnell,Lynn; Yu,Jianhua; Lu,Wu; Lee,L,James, 2015, "Dielectrophoresis-assisted 3D nanoelectroporation for non-viral cell transfection in adoptive immunotherapy." LAB ON A CHIP 15, no. 15, 3147 - 3153.

2014

  • Xie,Pengcheng; He,Peng; Yen,Ying-Chieh; Kwak,Kwang,Joo; Gallego-Perez,Daniel; Chang,Lingqian; Liao,Wei-Ching; Yi,Allen; Lee,L,James, 2014, "Rapid hot embossing of polymer microstructures using carbide-bonded graphene coating on silicon stampers." SURFACE & COATINGS TECHNOLOGY 258, 174 - 180.
  • Benavente-Babace,A; Gallego-Perez,D; Hansford,D,J; Arana,S; Perez-Lorenzo,E; Mujika,M, 2014, "Single-cell trapping and selective treatment via co-flow within a microfluidic platform." BIOSENSORS & BIOELECTRONICS 61, 298 - 305.
  • Gupta,C; Liao,W-C; Gallego-Perez,D; Castro,C,E; Lee,L,J, 2014, "DNA translocation through short nanofluidic channels under asymmetric pulsed electric field." BIOMICROFLUIDICS 8, no. 2, 024114 -
  • Wang,Xinmei; Huang,Xiaomeng; Yang,Zhaogang; Gallego-Perez,Daniel; Ma,Junyu; Zhao,Xi; Xie,Jing; Nakano,Ichiro; Lee,L,James, 2014, "Targeted Delivery of Tumor Suppressor microRNA-1 by Transferrin- Conjugated Lipopolyplex Nanoparticles to Patient-Derived Glioblastoma Stem Cells." CURRENT PHARMACEUTICAL BIOTECHNOLOGY 15, no. 9, 839 - 846.

2013

  • Wu,Yun; Terp,Megan,Cavanaugh; Kwak,Kwang,Joo; Gallego-Perez,Daniel; Nana-Sinkam,Serge,P; Lee,L,James, 2013, "Surface-Mediated Nucleic Acid Delivery by Lipoplexes Prepared in Microwell Arrays." SMALL 9, no. 13, 2358 - 2367.
  • Fei,Zhengzheng; Wu,Yun; Sharma,Sadhana; Gallego-Perez,Daniel; Higuita-Castro,Natalia; Hansford,Derek; Lannutti,John,J; Lee,Ly,James, 2013, "Gene Delivery to Cultured Embryonic Stem Cells Using Nanofiber-Based Sandwich Electroporation." ANALYTICAL CHEMISTRY 85, no. 3, 1401 - 1407.
  • Pelaez-Vargas,Alejandro; Gallego-Perez,Daniel; Carvalho,Angela; Fernandes,Maria,H; Hansford,Derek,J; Monteiro,Fernando,J, 2013, "Effects of density of anisotropic microstamped silica thin films on guided bone tissue regenerationIn vitro study." JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 101B, no. 5, 762 - 769.
  • Pelaez-Vargas,Alejandro; Gallego-Perez,Daniel; Carvalho,Angela; Fernandes,Maria,H; Hansford,Derek,J; Monteiro,Fernando,J, 2013, "Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration--in vitro study.." Journal of biomedical materials research. Part B, Applied biomaterials 101, no. 5, 762 - 769.
  • DINAN,B; Gallego-Perez,D; LEE,H; Hansford,D; Akbar,S,A, 2013, "Thermally grown TiO2 nanowires to improve cell growth and proliferation on titanium based materials." CERAMICS INTERNATIONAL 39, no. 5, 5949 - 5954.
  • Huang,Wenyi; Yu,Jianfeng; Kwak,Kwang,Joo; Gallego-Perez,Daniel; Liao,Wei-Ching; Yang,Hao; Ouyang,Xilian; Li,Lei; Lu,Wu; Lafyatis,Gregory,P; Lee,L,James, 2013, "Atomic Carbide Bonding Leading to Superior Graphene Networks." ADVANCED MATERIALS 25, no. 33, 4668 - 4672.

2012

  • Carvalho,A; Pelaez-Vargas,A; Gallego-Perez,D; Grenho,L; Fernandes,M,H; De Aza,A,H; Ferraz,M,P; Hansford,D,J; Monteiro,F,J, 2012, "Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration." DENTAL MATERIALS 28, no. 12, 1250 - 1260.
  • Wang,Feng; He,Hongyan; Wang,Xinmei; Li,Zhenqing; Gallego-Perez,Daniel; Guan,Jianjun; Lee,L,James, 2012, "Micropatterned Thermoresponsive Surfaces by Polymerization of Monomer Crystals: Modulating Cellular Morphology and Cell Substrate Interactions." ANALYTICAL CHEMISTRY 84, no. 21, 9439 - 9445.
  • Gallego-Perez,Daniel; Higuita-Castro,Natalia; Denning,Lisa; Dejesus,Jessica; Dahl,Kirstin; Sarkar,Atom; Hansford,Derek,J, 2012, "Microfabricated mimics of in vivo structural cues for the study of guided tumor cell migration." LAB ON A CHIP 12, no. 21, 4424 - 4432.
  • Higuita-Castro,Natalia; Gallego-Perez,Daniel; Pelaez-Vargas,Alejandro; Garcia Quiroz,Felipe; Posada,Olga,M; Lopez,Luis,E; Sarassa,Carlos,A; Agudelo-Florez,Piedad; Monteiro,Fernando,J; Litsky,Alan,S; Hansford,Derek,J, 2012, "Reinforced Portland cement porous scaffolds for load-bearing bone tissue engineering applications." JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 100B, no. 2, 501 - 507.
  • Gallego-Perez,Daniel; Higuita-Castro,Natalia; Reen,Rashmeet,K; Palacio-Ochoa,Marcela; Sharma,Sadhana; Lee,L,James; Lannutti,John,J; Hansford,Derek,J; Gooch,Keith,J, 2012, "Micro/nanoscale technologies for the development of hormone-expressing islet-like cell clusters." BIOMEDICAL MICRODEVICES 14, no. 4, 779 - 789.
  • Higuita-Castro,Natalia; Gallego-Perez,Daniel; Pelaez-Vargas,Alejandro; García Quiroz,Felipe; Posada,Olga,M; López,Luis,E; Sarassa,Carlos,A; Agudelo-Florez,Piedad; Monteiro,Fernando,J; Litsky,Alan,S; Hansford,Derek,J, 2012, "Reinforced Portland cement porous scaffolds for load-bearing bone tissue engineering applications.." Journal of biomedical materials research. Part B, Applied biomaterials 100, no. 2, 501 - 507.

2011

  • Gallego-Perez,Daniel; Higuita-Castro,Natalia; Quiroz,Felipe,García; Posada,Olga,M; López,Luis,E; Litsky,Alan,S; Hansford,Derek,J, 2011, "Portland cement for bone tissue engineering: Effects of processing and metakaolin blends.." Journal of biomedical materials research. Part B, Applied biomaterials 98, no. 2, 308 - 315.
  • Gallego-Perez,Daniel; Higuita-Castro,Natalia; Garcia Quiroz,Felipe; Posada,Olga,M; Lopez,Luis,E; Litsky,Alan,S; Hansford,Derek,J, 2011, "Portland cement for bone tissue engineering: Effects of processing and metakaolin blends." JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 98B, no. 2, 308 - 315.
  • Pelaez-Vargas,A; Gallego-Perez,D; Magallanes-Perdomo,M; Fernandes,M,H; Hansford,D,J; De Aza,A,H; Pena,P; Monteiro,F,J, 2011, "Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants." DENTAL MATERIALS 27, no. 6, 581 - 589.

2010

  • Pelaez-Vargas,A; Gallego-Perez,D; Ferrell,N; Fernandes,M,H; Hansford,D; Monteiro,F,J, 2010, "Early Spreading and Propagation of Human Bone Marrow Stem Cells on Isotropic and Anisotropic Topographies of Silica Thin Films Produced via Microstamping." MICROSCOPY AND MICROANALYSIS 16, no. 6, 670 - 676.
  • Ferrell,Nicholas; Gallego-Perez,Daniel; Higuita-Castro,Natalia; Butler,Randall,T; Reen,Rashmeet,K; Gooch,Keith,J; Hansford,Derek,J, 2010, "Vacuum-Assisted Cell Seeding in a Microwell Cell Culture System." ANALYTICAL CHEMISTRY 82, no. 6, 2380 - 2386.
  • Sabbani,Supriya; Gallego-Perez,Daniel; Nagy,Amber; Waldman,W,James; Hansford,Derek; Dutta,Prabir,K, 2010, "Synthesis of silver-zeolite films on micropatterned porous alumina and its application as an antimicrobial substrate." MICROPOROUS AND MESOPOROUS MATERIALS 135, no. 1-3, 131 - 136.
  • Gallego-Perez,Daniel; Ferrell,Nicholas,J; Higuita-Castro,Natalia; Hansford,Derek,J, 2010, "Versatile methods for the fabrication of polyvinylidene fluoride microstructures." BIOMEDICAL MICRODEVICES 12, no. 6, 1009 - 1017.
  • Ferrell,Nicholas; Woodard,James; Gallego-Perez,Daniel; Higuita-Castro,Natalia; Hansford,Derek, 2010, "POLYMER MEMS FOR MEASURING SINGLE CELL FORCES." PROCEEDINGS OF THE ASME INTERNATIONAL DESIGN ENGINEERING TECHNICAL CONFERENCES AND COMPUTERS AND INFORMATION IN ENGINEERING CONFERENCE, DETC 2010, VOL 4 4, 453 - 459.
  • Gallego-Perez,Daniel; Higuita-Castro,Natalia; Sharma,Sadhana; Reen,Rashmeet,K; Palmer,Andre,F; Gooch,Keith,J; Lee,L,James; Lannutti,John,J; Hansford,Derek,J, 2010, "High throughput assembly of spatially controlled 3D cell clusters on a micro/nanoplatform." LAB ON A CHIP 10, no. 6, 775 - 782.
  • Garcia Quiroz,Felipe; Posada,Olga,M; Gallego-Perez,Daniel; Higuita-Castro,Natalia; Sarassa,Carlos; Hansford,Derek,J; Agudelo-Florez,Piedad; Lopez,Luis,E, 2010, "Housekeeping gene stability influences the quantification of osteogenic markers during stem cell differentiation to the osteogenic lineage." CYTOTECHNOLOGY 62, no. 2, 109 - 120.

2009

  • Xu,Jian; Dapino,Marcelo,J; Perez,Daniel,Gallego; Hansford,Derek, 2009, "ACOUSTIC SENSOR WITH PVDF MICRO-PILLARS AND PATTERNED ELECTRODES." SMASIS2008: PROCEEDINGS OF THE ASME CONFERENCE ON SMART MATERIALS, ADAPTIVE STRUCTURES AND INTELLIGENT SYSTEMS - 2008, VOL 1 721 - 727.
  • Xu,J; Dapino,M,J; Gallego-Perez,D; Hansford,D, 2009, "Microphone based on Polyvinylidene Fluoride (PVDF) micro-pillars and patterned electrodes." SENSORS AND ACTUATORS A-PHYSICAL 153, no. 1, 24 - 32.

2008

  • Gallego,Daniel; Higuita,Natalia; Garcia,Felipe; Ferrell,Nicholas; Hansford,Derek,J, 2008, "Bioactive coatings on Portland cement substrates: Surface precipitation of apatite-like crystals." MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 28, no. 3, 347 - 352.
  • Gallego,Daniel; Ferrell,Nicholas; Sun,Yang; Hansford,Derek,J, 2008, "Multilayer micromolding of degradable polymer tissue engineering scaffolds." MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 28, no. 3, 353 - 358.