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Daniel Gallego Perez

  • Assistant Professor, General Surgery
  • Assistant Professor, Biomedical Engineering
  • Davis Heart and Lung Research Institute
    473 W 12th Ave, Room 012E/F
    Columbus, OH 43210
  • 614-685-0672



  • B.S., Antioquia School of Engineering, 2003
  • Ph.D., The Ohio State University, 2011


Key Honors and Distinctions

  • Lumley Interdisciplinary Research Award, 2017.
  • William G. Lowrie Department of Chemical and Biomolecular Engineering, Outstanding Post-Doc Award for Research Excellence, 2014.
  • CWRU Society for Biomaterials – Biomaterials Day, Student Presentation Award, 2010.
  • Ohio State Biomedical Engineering Conference, Best Presentation in Micro/Nanotechnology, 2009.
  • Society for Biomaterials Meeting, Student Travel Achievement Recognition, 2009.
  • Biomedical Engineering Society Meeting, Student Travel Award, 2008.
  • Ohio State Biomedical Engineering Conference, Best Presentation in Micro/Nanotechnology, 2008.
  • The 8th New Jersey Symposium on Biomaterials Science, Student Travel Scholarship, 2006.


RESEARCH AREAS – Gallego-Perez NanoMedicine Lab

  • Lab-on-a-Chip platforms, single cell analyses, gene delivery, cell reprogramming
  • Graduate student research opportunities are currently available. [indicate whether you have grad student or postdoc opportunities in the lab][D1] Open positions (




    • Hansford D, Schley J, Gallego-Perez D. “Electroosmotic devices for fluid handling” US Patent #61/883,592. 9/27/13.
    • Pal D, Gallego-Perez D, Ghatak S, Lee LJ, Sen CK. “compositions and methods for reprogramming somatic cells into induced endothelial cells”. Provisional patent filed (2016).

The Nano Medicine Laboratory at OSU is dedicated to the development and implementation of novel micro-/nanoscale platforms (e.g., Lab-on-Chip devices, gene delivery chips, tissue engineering scaffolds) for applications in fundamental and/or translational medical research.



  • 2015. "Microwell Array-Mediated Delivery of Lipoplexes Containing Nucleic Acids for Enhanced Therapeutic Efficacy." In RNA interference: challenges and therapeutic opportunities, edited by Sioud, M,

Journal Articles


  • Shukla, V.C.; Kuang, T-R.; Senthilvelan, A.; Higuita-Castro, N. et al., 2018, "Lab-on-a-Chip Platforms for Biophysical Studies of Cancer with Single-Cell Resolution.." Trends Biotechnol 36, no. 5, 549-561 - 549-561.


  • Kuang, T.; Chang, L.; Peng, X.; Hu, X. et al., 2017, "Molecular Beacon Nano-Sensors for Probing Living Cancer Cells.." Trends Biotechnol 35, no. 4, 347-359 - 347-359.


  • Chang, L.; Li, L.; Shi, J.; Sheng, Y. et al., 2016, "Micro-/nanoscale electroporation.." Lab Chip 16, no. 21, 4047-4062 - 4047-4062.
  • Gallego-Perez, D.; Chang, L.; Shi, J.; Ma, J. et al., 2016, "On-Chip Clonal Analysis of Glioma-Stem-Cell Motility and Therapy Resistance.." Nano Lett 16, no. 9, 5326-5332 - 5326-5332.
  • Chang, L.; Bertani, P.; Gallego-Perez, D.; Yang, Z. et al., 2016, "3D nanochannel electroporation for high-throughput cell transfection with high uniformity and dosage control.." Nanoscale 8, no. 1, 243-252 - 243-252.
  • Gu, S.Q.; Gallego-Perez, D.; McClory, S.P.; Shi, J. et al., 2016, "The human PMR1 endonuclease stimulates cell motility by down regulating miR-200 family microRNAs." Nucleic Acids Research 44, no. 12, 5811-5819 - 5811-5819.
  • Chang, L.; Gallego-Perez, D.; Chiang, C-L.; Bertani, P. et al., 2016, "Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform.." Small 12, no. 43, 5971-5980 - 5971-5980.
  • Kim, S-H.; Ezhilarasan, R.; Phillips, E.; Gallego-Perez, D. et al., 2016, "Serine/Threonine Kinase MLK4 Determines Mesenchymal Identity in Glioma Stem Cells in an NF-κB-dependent Manner.." Cancer Cell 29, no. 2, 201-213 - 201-213.
  • Gallego-Perez, D.; Otero, J.J.; Czeisler, C.; Ma, J. et al., 2016, "Deterministic transfection drives efficient nonviral reprogramming and uncovers reprogramming barriers.." Nanomedicine 12, no. 2, 399-409 - 399-409.


  • Holfinger, S.J.; Reinhardt, J.W.; Reen, R.; Schultz, K.M. et al., 2015, "Pancreatic Epithelial Cells Form Islet-Like Clusters in the Absence of Directed Migration." Cellular and Molecular Bioengineering 8, no. 3, 496-506 - 496-506.
  • Malkoc, V.; Gallego-Perez, D.; Nelson, T.; Lannutti, J.J. et al., 2015, "Controlled neuronal cell patterning and guided neurite growth on micropatterned nanofiber platforms." Journal of Micromechanics and Microengineering 25, no. 12,
  • Czeisler, C.; Gallego-Perez, D.; Ortiz, C.; Gygli, P. et al., 2015, "Nuclear Reprogramming with Hindbrain Patterning Genes by Nanochannel Electroporation." FASEB JOURNAL 29,
  • Bertani, P.; Lu, W.; Chang, L.; Gallego-Perez, D. et al., 2015, "Bosch etching for the creation of a 3D nanoelectroporation system for high throughput gene delivery." JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B 33, no. 6,
  • Zhao, X.; Huang, X.; Wang, X.; Wu, Y. et al., 2015, "Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.." Adv Sci (Weinh) 2, no. 12, 1500111 - 1500111.
  • Zhao, X.; Wu, Y.; Gallego-Perez, D.; Kwak, K.J. et al., 2015, "Effect of nonendocytic uptake of nanoparticles on human bronchial epithelial cells.." Anal Chem 87, no. 6, 3208-3215 - 3208-3215.
  • Chang, L.; Gallego-Perez, D.; Zhao, X.; Bertani, P. et al., 2015, "Dielectrophoresis-assisted 3D nanoelectroporation for non-viral cell transfection in adoptive immunotherapy.." Lab Chip 15, no. 15, 3147-3153 - 3147-3153.
  • Chang, L.; Howdyshell, M.; Liao, W-C.; Chiang, C-L. et al., 2015, "Magnetic tweezers-based 3D microchannel electroporation for high-throughput gene transfection in living cells.." Small 11, no. 15, 1818-1828 - 1818-1828.


  • Gupta, C.; Liao, W-C.; Gallego-Perez, D.; Castro, C.E. et al., 2014, "DNA translocation through short nanofluidic channels under asymmetric pulsed electric field.." Biomicrofluidics 8, no. 2, 024114 - 024114.
  • Wang, X.; Huang, X.; Yang, Z.; Gallego-Perez, D. et al., 2014, "Targeted delivery of tumor suppressor microRNA-1 by transferrin-conjugated lipopolyplex nanoparticles to patient-derived glioblastoma stem cells.." Curr Pharm Biotechnol 15, no. 9, 839-846 - 839-846.
  • Benavente-Babace, A.; Gallego-Pérez, D.; Hansford, D.J.; Arana, S. et al., 2014, "Single-cell trapping and selective treatment via co-flow within a microfluidic platform.." Biosens Bioelectron 61, 298-305 - 298-305.
  • Xie, P.; He, P.; Yen, Y.C.; Kwak, K.J. et al., 2014, "Rapid hot embossing of polymer microstructures using carbide-bonded graphene coating on silicon stampers." Surface and Coatings Technology 258, 174-180 - 174-180.


  • Wu, Y.; Terp, M.C.; Kwak, K.J.; Gallego-Perez, D. et al., 2013, "Surface-mediated nucleic acid delivery by lipoplexes prepared in microwell arrays.." Small 9, no. 13, 2358-2367 - 2358-2367.
  • Fei, Z.; Wu, Y.; Sharma, S.; Gallego-Perez, D. et al., 2013, "Gene delivery to cultured embryonic stem cells using nanofiber-based sandwich electroporation.." Anal Chem 85, no. 3, 1401-1407 - 1401-1407.
  • Dinan, B.; Gallego-Perez, D.; Lee, H.; Hansford, D. et al., 2013, "Thermally grown TiO2 nanowires to improve cell growth and proliferation on titanium based materials." Ceramics International 39, no. 5, 5949-5954 - 5949-5954.
  • Huang, W.; Yu, J.; Kwak, K.J.; Gallego-Perez, D. et al., 2013, "Atomic carbide bonding leading to superior graphene networks.." Adv Mater 25, no. 33, 4668-4672 - 4668-4672.
  • Pelaez-Vargas, A.; Gallego-Perez, D.; Carvalho, A.; Fernandes, M.H. et al., 2013, "Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration--in vitro study.." J Biomed Mater Res B Appl Biomater 101, no. 5, 762-769 - 762-769.


  • Gallego-Perez, D.; Higuita-Castro, N.; Denning, L.; DeJesus, J. et al., 2012, "Microfabricated mimics of in vivo structural cues for the study of guided tumor cell migration.." Lab Chip 12, no. 21, 4424-4432 - 4424-4432.
  • Higuita-Castro, N.; Gallego-Perez, D.; Pelaez-Vargas, A.; García Quiroz, F. et al., 2012, "Reinforced Portland cement porous scaffolds for load-bearing bone tissue engineering applications.." J Biomed Mater Res B Appl Biomater 100, no. 2, 501-507 - 501-507.
  • Carvalho, A.; Pelaez-Vargas, A.; Gallego-Perez, D.; Grenho, L. et al., 2012, "Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration.." Dent Mater 28, no. 12, 1250-1260 - 1250-1260.
  • Gallego-Perez, D.; Higuita-Castro, N.; Reen, R.K.; Palacio-Ochoa, M. et al., 2012, "Micro/nanoscale technologies for the development of hormone-expressing islet-like cell clusters.." Biomed Microdevices 14, no. 4, 779-789 - 779-789.
  • Wang, F.; He, H.; Wang, X.; Li, Z. et al., 2012, "Micropatterned thermoresponsive surfaces by polymerization of monomer crystals: modulating cellular morphology and cell-substrate interactions.." Anal Chem 84, no. 21, 9439-9445 - 9439-9445.


  • Pelaez-Vargas, A.; Gallego-Perez, D.; Magallanes-Perdomo, M.; Fernandes, M.H. et al., 2011, "Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants.." Dent Mater 27, no. 6, 581-589 - 581-589.
  • Gallego-Perez, D.; Higuita-Castro, N.; Quiroz, F.G.; Posada, O.M. et al., 2011, "Portland cement for bone tissue engineering: Effects of processing and metakaolin blends.." J Biomed Mater Res B Appl Biomater 98, no. 2, 308-315 - 308-315.


  • Quiroz, F.G.; Posada, O.M.; Gallego-Perez, D.; Higuita-Castro, N. et al., 2010, "Housekeeping gene stability influences the quantification of osteogenic markers during stem cell differentiation to the osteogenic lineage.." Cytotechnology 62, no. 2, 109-120 - 109-120.
  • Gallego-Perez, D.; Ferrell, N.J.; Higuita-Castro, N.; Hansford, D.J., 2010, "Versatile methods for the fabrication of polyvinylidene fluoride microstructures.." Biomed Microdevices 12, no. 6, 1009-1017 - 1009-1017.
  • Gallego-Perez, D.; Higuita-Castro, N.; Sharma, S.; Reen, R.K. et al., 2010, "High throughput assembly of spatially controlled 3D cell clusters on a micro/nanoplatform.." Lab Chip 10, no. 6, 775-782 - 775-782.
  • Ferrell, N.; Gallego-Perez, D.; Higuita-Castro, N.; Butler, R.T. et al., 2010, "Vacuum-assisted cell seeding in a microwell cell culture system.." Anal Chem 82, no. 6, 2380-2386 - 2380-2386.
  • Sabbani, S.; Gallego-Perez, D.; Nagy, A.; James Waldman, W. et al., 2010, "Synthesis of silver-zeolite films on micropatterned porous alumina and its application as an antimicrobial substrate." Microporous and Mesoporous Materials 135, no. 1-3, 131-136 - 131-136.
  • Pelaez-Vargas, A.; Gallego-Perez, D.; Ferrell, N.; Fernandes, M.H. et al., 2010, "Early spreading and propagation of human bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping.." Microsc Microanal 16, no. 6, 670-676 - 670-676.


  • Xu, J.; Dapino, M.J.; Gallego-Perez, D.; Hansford, D., 2009, "Microphone based on Polyvinylidene Fluoride (PVDF) micro-pillars and patterned electrodes." Sensors and Actuators, A: Physical 153, no. 1, 24-32 - 24-32.


  • Xu, J.; Dapino, M.J.; Perez, D.G.; Hansford, D., 2008, "Acoustic sensor with PVDF micro-pillars and patterned electrodes." Proceedings of the ASME Conference on Smart Materials, Adaptive Structures and Intelligent Systems, SMASIS2008 1, 721-727 - 721-727.
  • Gallego, D.; Ferrell, N.; Sun, Y.; Hansford, D.J., 2008, "Multilayer micromolding of degradable polymer tissue engineering scaffolds." Materials Science and Engineering C 28, no. 3, 353-358 - 353-358.


  • "Fabrication methods for polymer MEMS." 2007, Presented at American society for precision engineering,

Papers in Proceedings


  • Gygli, P.E.; Byers, S.; Higuita-Castro, N.; Czeisler, C. et al. "Deterministic transfection and genetic manipulation of organotypic brain slice cultures for ex vivo imaging." (4 2016).


  • Bertani, P.; Chang, L.Q.; Gallego-Perez, D.; Malkoc, V. et al. "3D Si-based nanochannel platform for robust cell electroporation." (8 2015).


  • Pelaez-Vargas, A.; Gallego-Perez, D.; Fernandes, M.H.; Hansford, D. et al. "Microstructured coatings to study the behavior of osteoblast-like cells on hard materials." (5 2011).
  • Carvalho, A.; Pelaez-Vargas, A.; Gallego-Perez, D.; Fernandes, M.H. et al. "Adhesion and proliferation of mesenchymal stem cells on micropatterned thin films modified with nanohydroxyapatite particles." (5 2011).


  • Xu, J.; Dapino, M.J.; Gallego-Perez, D.; Hansford, D. "Validation and characterization of an acoustic sensor based on PVDF micropillars and patterned electrodes." (12 2010).
  • Ferrell,Nicholas; Woodard,James; Gallego-Perez,Daniel; Higuita-Castro,Natalia; Hansford,Derek "POLYMER MEMS FOR MEASURING SINGLE CELL FORCES." (1 2010).


  • D Gallego-Perez, N Ferrell, DJ Hansford "Fabrication of piezoelectric Polyvinylidene Fluoride (PVDF) microstructures by soft lithography for tissue engineering and cell biology applications." in MRS Spring Meeting 2007. (4 2007).


  • D Gallego-Perez, N Higuita-Castro, F Garcia, N Ferrell, DJ Hansford "Bioactive coatings on Portland cement: Surface precipitation of apatite-like crystals." in Symposium on Advanced Processing of Biomaterials. (10 2006).