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  • Daniel Gallego-Perez

Daniel Gallego Perez

  • Assistant Professor, General Surgery
  • Assistant Professor, Biomedical Engineering
  • Davis Heart and Lung Research Institute
    473 W 12th Ave, Room 012E/F
    Columbus, OH 43210
  • 614-685-0672

About

Education

  • B.S., Antioquia School of Engineering, 2003
  • Ph.D., The Ohio State University, 2011

 

Key Honors and Distinctions

  • Lumley Interdisciplinary Research Award, 2017.
  • William G. Lowrie Department of Chemical and Biomolecular Engineering, Outstanding Post-Doc Award for Research Excellence, 2014.
  • CWRU Society for Biomaterials – Biomaterials Day, Student Presentation Award, 2010.
  • Ohio State Biomedical Engineering Conference, Best Presentation in Micro/Nanotechnology, 2009.
  • Society for Biomaterials Meeting, Student Travel Achievement Recognition, 2009.
  • Biomedical Engineering Society Meeting, Student Travel Award, 2008.
  • Ohio State Biomedical Engineering Conference, Best Presentation in Micro/Nanotechnology, 2008.
  • The 8th New Jersey Symposium on Biomaterials Science, Student Travel Scholarship, 2006.

 

RESEARCH AREAS – Gallego-Perez NanoMedicine Lab https://gallegopereznanomed.engineering.osu.edu/

  • Lab-on-a-Chip platforms, single cell analyses, gene delivery, cell reprogramming
  • Graduate student research opportunities are currently available. [indicate whether you have grad student or postdoc opportunities in the lab][D1] Open positions (https://gallegopereznanomed.engineering.osu.edu/)

 

  • PUBLICATIONS

https://scholar.google.com/citations?user=aXJcCFgAAAAJ&hl=en

 

  • PATENTS
    • Hansford D, Schley J, Gallego-Perez D. “Electroosmotic devices for fluid handling” US Patent #61/883,592. 9/27/13.
    • Pal D, Gallego-Perez D, Ghatak S, Lee LJ, Sen CK. “compositions and methods for reprogramming somatic cells into induced endothelial cells”. Provisional patent filed (2016).

The Nano Medicine Laboratory at OSU is dedicated to the development and implementation of novel micro-/nanoscale platforms (e.g., Lab-on-Chip devices, gene delivery chips, tissue engineering scaffolds) for applications in fundamental and/or translational medical research.

Honors

  • Institute for Materials Research Innovation Award.

  • Lumley Interdisciplinary Research Award.

  • Outstanding Post-Doc Award for Research Excellence.

  • Student Presentation Award.

  • Best Presentation in Micro/Nanotechnology.

  • Student Travel Achievement Recognition.

  • Student Travel Award.

  • Student Travel Scholarship.

Chapters

2015

  • 2015. "Microwell Array-Mediated Delivery of Lipoplexes Containing Nucleic Acids for Enhanced Therapeutic Efficacy." In RNA interference: challenges and therapeutic opportunities, edited by Sioud, M,
  • Wu,Yun; Gallego-Perez,Daniel; Lee,L,James. 2015. "Microwell array-mediated delivery of lipoplexes containing nucleic acids for enhanced therapeutic efficacy.."

2012

  • 2012. "Micropatterned Coatings for Guided Tissue Regeneration in Dental Implantology." In Cell Interaction,
  • 2012. "Propagation of Human Bone Marrow Stem Cells for Craniofacial Applications." In Stem Cells and Cancer Stem Cells: Therapeutic Applications in Disease and Injury,

Journal Articles

2018

  • Shukla, V.C.; Kuang, T-R.; Senthilvelan, A.; Higuita-Castro, N. et al., 2018, "Lab-on-a-Chip Platforms for Biophysical Studies of Cancer with Single-Cell Resolution.." Trends Biotechnol 36, no. 5, 549-561 - 549-561.

2017

  • Kuang, T.; Chang, L.; Peng, X.; Hu, X. et al., 2017, "Molecular Beacon Nano-Sensors for Probing Living Cancer Cells.." Trends Biotechnol 35, no. 4, 347-359 - 347-359.
  • Gallego Perez, D.; Pal, D.; Ghatak, S.; Malkoc, V. et al., 2017, "Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue." Nature Nanotechnology 974-979 - 974-979.

2016

  • Chang, L.; Li, L.; Shi, J.; Sheng, Y. et al., 2016, "Micro-/nanoscale electroporation.." Lab Chip 16, no. 21, 4047-4062 - 4047-4062.
  • Gallego-Perez, D.; Chang, L.; Shi, J.; Ma, J. et al., 2016, "On-Chip Clonal Analysis of Glioma-Stem-Cell Motility and Therapy Resistance.." Nano Lett 16, no. 9, 5326-5332 - 5326-5332.
  • Chang, L.; Bertani, P.; Gallego-Perez, D.; Yang, Z. et al., 2016, "3D nanochannel electroporation for high-throughput cell transfection with high uniformity and dosage control.." Nanoscale 8, no. 1, 243-252 - 243-252.
  • Gu, S.Q.; Gallego-Perez, D.; McClory, S.P.; Shi, J. et al., 2016, "The human PMR1 endonuclease stimulates cell motility by down regulating miR-200 family microRNAs." Nucleic Acids Research 44, no. 12, 5811-5819 - 5811-5819.
  • Chang, L.; Gallego-Perez, D.; Chiang, C-L.; Bertani, P. et al., 2016, "Controllable Large-Scale Transfection of Primary Mammalian Cardiomyocytes on a Nanochannel Array Platform.." Small 12, no. 43, 5971-5980 - 5971-5980.
  • Kim, S-H.; Ezhilarasan, R.; Phillips, E.; Gallego-Perez, D. et al., 2016, "Serine/Threonine Kinase MLK4 Determines Mesenchymal Identity in Glioma Stem Cells in an NF-κB-dependent Manner.." Cancer Cell 29, no. 2, 201-213 - 201-213.
  • Gallego-Perez, D.; Otero, J.J.; Czeisler, C.; Ma, J. et al., 2016, "Deterministic transfection drives efficient nonviral reprogramming and uncovers reprogramming barriers.." Nanomedicine 12, no. 2, 399-409 - 399-409.

2015

  • Holfinger, S.J.; Reinhardt, J.W.; Reen, R.; Schultz, K.M. et al., 2015, "Pancreatic Epithelial Cells Form Islet-Like Clusters in the Absence of Directed Migration." Cellular and Molecular Bioengineering 8, no. 3, 496-506 - 496-506.
  • Malkoc, V.; Gallego-Perez, D.; Nelson, T.; Lannutti, J.J. et al., 2015, "Controlled neuronal cell patterning and guided neurite growth on micropatterned nanofiber platforms." Journal of Micromechanics and Microengineering 25, no. 12,
  • Czeisler, C.; Gallego-Perez, D.; Ortiz, C.; Gygli, P. et al., 2015, "Nuclear Reprogramming with Hindbrain Patterning Genes by Nanochannel Electroporation." FASEB JOURNAL 29,
  • Bertani, P.; Lu, W.; Chang, L.; Gallego-Perez, D. et al., 2015, "Bosch etching for the creation of a 3D nanoelectroporation system for high throughput gene delivery." JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B 33, no. 6,
  • Zhao, X.; Huang, X.; Wang, X.; Wu, Y. et al., 2015, "Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.." Adv Sci (Weinh) 2, no. 12, 1500111 - 1500111.
  • Zhao, X.; Wu, Y.; Gallego-Perez, D.; Kwak, K.J. et al., 2015, "Effect of nonendocytic uptake of nanoparticles on human bronchial epithelial cells.." Anal Chem 87, no. 6, 3208-3215 - 3208-3215.
  • Chang, L.; Gallego-Perez, D.; Zhao, X.; Bertani, P. et al., 2015, "Dielectrophoresis-assisted 3D nanoelectroporation for non-viral cell transfection in adoptive immunotherapy.." Lab Chip 15, no. 15, 3147-3153 - 3147-3153.
  • Chang, L.; Howdyshell, M.; Liao, W-C.; Chiang, C-L. et al., 2015, "Magnetic tweezers-based 3D microchannel electroporation for high-throughput gene transfection in living cells.." Small 11, no. 15, 1818-1828 - 1818-1828.

2014

  • Wang, X.; Huang, X.; Yang, Z.; Gallego-Perez, D. et al., 2014, "Targeted delivery of tumor suppressor microRNA-1 by transferrin-conjugated lipopolyplex nanoparticles to patient-derived glioblastoma stem cells.." Curr Pharm Biotechnol 15, no. 9, 839-846 - 839-846.
  • Benavente-Babace, A.; Gallego-Pérez, D.; Hansford, D.J.; Arana, S. et al., 2014, "A Versatile Microfabricated Platform for Single-Cell Studies." IFMBE Proceedings 41, 864-867 - 864-867.
  • Gupta, C.; Liao, W-C.; Gallego-Perez, D.; Castro, C.E. et al., 2014, "DNA translocation through short nanofluidic channels under asymmetric pulsed electric field.." Biomicrofluidics 8, no. 2, 024114 - 024114.
  • Benavente-Babace, A.; Gallego-Pérez, D.; Hansford, D.J.; Arana, S. et al., 2014, "Single-cell trapping and selective treatment via co-flow within a microfluidic platform.." Biosens Bioelectron 61, 298-305 - 298-305.
  • Xie, P.; He, P.; Yen, Y.C.; Kwak, K.J. et al., 2014, "Rapid hot embossing of polymer microstructures using carbide-bonded graphene coating on silicon stampers." Surface and Coatings Technology 258, 174-180 - 174-180.

2013

  • Fei, Z.; Wu, Y.; Sharma, S.; Gallego-Perez, D. et al., 2013, "Gene delivery to cultured embryonic stem cells using nanofiber-based sandwich electroporation.." Anal Chem 85, no. 3, 1401-1407 - 1401-1407.
  • Wu, Y.; Terp, M.C.; Kwak, K.J.; Gallego-Perez, D. et al., 2013, "Surface-mediated nucleic acid delivery by lipoplexes prepared in microwell arrays.." Small 9, no. 13, 2358-2367 - 2358-2367.
  • Huang, W.; Yu, J.; Kwak, K.J.; Gallego-Perez, D. et al., 2013, "Atomic carbide bonding leading to superior graphene networks.." Adv Mater 25, no. 33, 4668-4672 - 4668-4672.
  • Pelaez-Vargas, A.; Gallego-Perez, D.; Carvalho, A.; Fernandes, M.H. et al., 2013, "Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration--in vitro study.." J Biomed Mater Res B Appl Biomater 101, no. 5, 762-769 - 762-769.
  • Dinan, B.; Gallego-Perez, D.; Lee, H.; Hansford, D. et al., 2013, "Thermally grown TiO2 nanowires to improve cell growth and proliferation on titanium based materials." Ceramics International 39, no. 5, 5949-5954 - 5949-5954.

2012

  • Gallego-Perez, D.; Higuita-Castro, N.; Denning, L.; DeJesus, J. et al., 2012, "Microfabricated mimics of in vivo structural cues for the study of guided tumor cell migration.." Lab Chip 12, no. 21, 4424-4432 - 4424-4432.
  • Higuita-Castro, N.; Gallego-Perez, D.; Pelaez-Vargas, A.; García Quiroz, F. et al., 2012, "Reinforced Portland cement porous scaffolds for load-bearing bone tissue engineering applications.." J Biomed Mater Res B Appl Biomater 100, no. 2, 501-507 - 501-507.
  • Wang, F.; He, H.; Wang, X.; Li, Z. et al., 2012, "Micropatterned thermoresponsive surfaces by polymerization of monomer crystals: modulating cellular morphology and cell-substrate interactions.." Anal Chem 84, no. 21, 9439-9445 - 9439-9445.
  • Carvalho, A.; Pelaez-Vargas, A.; Gallego-Perez, D.; Grenho, L. et al., 2012, "Micropatterned silica thin films with nanohydroxyapatite micro-aggregates for guided tissue regeneration.." Dent Mater 28, no. 12, 1250-1260 - 1250-1260.
  • Gallego-Perez, D.; Higuita-Castro, N.; Reen, R.K.; Palacio-Ochoa, M. et al., 2012, "Micro/nanoscale technologies for the development of hormone-expressing islet-like cell clusters.." Biomed Microdevices 14, no. 4, 779-789 - 779-789.

2011

  • Pelaez-Vargas, A.; Gallego-Perez, D.; Magallanes-Perdomo, M.; Fernandes, M.H. et al., 2011, "Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants.." Dent Mater 27, no. 6, 581-589 - 581-589.
  • Gallego-Perez, D.; Higuita-Castro, N.; Quiroz, F.G.; Posada, O.M. et al., 2011, "Portland cement for bone tissue engineering: Effects of processing and metakaolin blends.." J Biomed Mater Res B Appl Biomater 98, no. 2, 308-315 - 308-315.

2010

  • Gallego-Perez, D.; Ferrell, N.J.; Higuita-Castro, N.; Hansford, D.J., 2010, "Versatile methods for the fabrication of polyvinylidene fluoride microstructures.." Biomed Microdevices 12, no. 6, 1009-1017 - 1009-1017.
  • Pelaez-Vargas, A.; Gallego-Perez, D.; Ferrell, N.; Fernandes, M.H. et al., 2010, "Early spreading and propagation of human bone marrow stem cells on isotropic and anisotropic topographies of silica thin films produced via microstamping.." Microsc Microanal 16, no. 6, 670-676 - 670-676.
  • Gallego-Perez, D.; Higuita-Castro, N.; Sharma, S.; Reen, R.K. et al., 2010, "High throughput assembly of spatially controlled 3D cell clusters on a micro/nanoplatform.." Lab Chip 10, no. 6, 775-782 - 775-782.
  • Sabbani, S.; Gallego-Perez, D.; Nagy, A.; James Waldman, W. et al., 2010, "Synthesis of silver-zeolite films on micropatterned porous alumina and its application as an antimicrobial substrate." Microporous and Mesoporous Materials 135, no. 1-3, 131-136 - 131-136.
  • Ferrell, N.; Gallego-Perez, D.; Higuita-Castro, N.; Butler, R.T. et al., 2010, "Vacuum-assisted cell seeding in a microwell cell culture system.." Anal Chem 82, no. 6, 2380-2386 - 2380-2386.
  • Quiroz, F.G.; Posada, O.M.; Gallego-Perez, D.; Higuita-Castro, N. et al., 2010, "Housekeeping gene stability influences the quantification of osteogenic markers during stem cell differentiation to the osteogenic lineage.." Cytotechnology 62, no. 2, 109-120 - 109-120.

2009

  • Xu, J.; Dapino, M.J.; Gallego-Perez, D.; Hansford, D., 2009, "Microphone based on Polyvinylidene Fluoride (PVDF) micro-pillars and patterned electrodes." Sensors and Actuators, A: Physical 153, no. 1, 24-32 - 24-32.

2008

  • Xu, J.; Dapino, M.J.; Perez, D.G.; Hansford, D., 2008, "Acoustic sensor with PVDF micro-pillars and patterned electrodes." Proceedings of the ASME Conference on Smart Materials, Adaptive Structures and Intelligent Systems, SMASIS2008 1, 721-727 - 721-727.
  • Gallego, D.; Ferrell, N.; Sun, Y.; Hansford, D.J., 2008, "Multilayer micromolding of degradable polymer tissue engineering scaffolds." Materials Science and Engineering C 28, no. 3, 353-358 - 353-358.
  • Gallego,Daniel; Higuita,Natalia; Garcia,Felipe; Ferrell,Nicholas; Hansford,Derek,J, 2008, "Bioactive coatings on Portland cement substrates: Surface precipitation of apatite-like crystals." MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 28, no. 3, 347-352 - 347-352.

Presentations

  • "Ectopic development of neuronal organoids via direct tissue reprogramming. Society for Neuroscience." 2017, Presented at Society for Neuroscience,
  • "Nanotechnology-based non-viral approaches for gene delivery to peripheral nerves." 2017, Presented at Society for Neuroscience,
  • "Neurotrophic skin environment caused by direct reprograming of skin cells to neural cells may rescue against loss of neural fibers caused by diabetic peripheral neuropathy.." 2017, Presented at Society for Neuroscience,
  • "Nanotechnology-based Reprogramming of Diabetic Tissue Resolves Local Vasculopathy." 2017, Presented at Biomedical Engineering Society Meeting,
  • "Micro- and Nano-engineered Surfaces Modulate the Phenotype of Blood-derived Cells: Applications in Regenerative Medicine." 2017, Presented at Biomedical Engineering Society Meeting,
  • "Cells on Gels: Biomimetic Micron-Scale Polyacrylamide Gel Substrates for Studies of GBM Cell Adhesion and Migration." 2017, Presented at Biomedical Engineering Society Meeting,
  • "Using Alveolar Pneumocyte Self-Derived Exosomes to Modulate Lung Inflammatory Response." 2017, Presented at Biomedical Engineering Society Meeting,
  • "Guiding Tumor Cell Invasion using Non-soluble Cues in the Tumor Microenvironment." 2017, Presented at Biomedical Engineering Society Meeting,
  • "Non-Viral Gene Delivery to Peripheral Nerve through a Nanostructured Chip Platform." 2016, Presented at Biomedical Engineering Society Meeting,
  • "Inducing Tissue Plasticity and Repair via Nanochannel-mediated Gene Delivery." 2016, Presented at Biomedical Engineering Society Meeting,
  • "Probing interclonal heterogeneities in patient-derived glioma stem cell populations via micro/nanoscale technologies." 2015, Presented at Biomedical Engineering Society Meeting,
  • "Controlled tissue transdifferentiation by nanochannel electroporation." 2015, Presented at Biomedical Engineering Society Meeting,
  • "Nanotechnology-Based Approaches for Triggering and Controlling Skin Cell Reprogramming In Vivo." 2015, Presented at Military Health System Research Symposium,
  • "Nanochannel-Based Electrotransfection of Skin Cells In Vivo." 2014, Presented at Biomedical Engineering Society Meeting,
  • "Controlled Cell Transdifferentiation In Vitro by Nanochannel Electroporation." 2014, Presented at Biomedical Engineering Society Meeting,
  • "Probing the migratory behavior of patient-derived glioma stem cells on a micro-engineered platform - a single cell analysis." 2013, Presented at Biomedical Engineering Society Meeting,
  • "Direct conversion of fibroblast to neurons via nanochannel electroporation." 2013, Presented at Biomedical Engineering Society Meeting,
  • "Immuno-Lipoplex Nanoparticle Microarrays for Capture and Detection of Circulating Tumor Cells and Exosomes." 2012, Presented at Materials Research Society Meeting,
  • "Nanoelectroporation for safe and efficient cell reprogramming." 2012, Presented at Biomedical Engineering Society Meeting,
  • "On-chip capture/sorting and characterization of cancerous cells." 2012, Presented at Biomedical Engineering Society Meeting,
  • "CEBPA Mutant Regulates miR181a Expression in AML Cells: A Single Cell Study by Nanochannel Electroporation." 2012, Presented at AIChE meeting,
  • "Micro/Nanoengineering Hormone-Expressing Islet-Like Tissue Subunits." 2011, Presented at Biomedical Engineering Society Meeting,
  • "Guided Tumor Cell Migration on Microscale Mimics of Contact Guidance Cues." 2011, Presented at Biomedical Engineering Society Meeting,
  • "Micropatterning Neuronal Networks on Nanofibers Platform." 2011, Presented at Biomedical Engineering Society Meeting,
  • "Adhesion and proliferation of mesenchymal stem cells on micropatterned thin films modified with nanohydroxyapatite particles." 2011, Presented at 3rd Joint Meeting of the European Calcified Tissue Society & the International Bone and Mineral Society,
  • "Microstructured coatings to study the behavior of osteoblast-like cells on hard materials." 2011, Presented at 3rd Joint Meeting of the European Calcified Tissue Society & the International Bone and Mineral Society,
  • "Guided Assembly of Insulin-Expressing Islet-Like Cell clusters on Polymeric Micro/Nanowells." 2010, Presented at Biomedical Engineering Society Meeting,
  • "Micropatterning and Printing of Functional Microscale Tissue Subunits on Nanofibrous Scaffolds." 2010, Presented at Biomedical Engineering Society Meeting,
  • "Evaluation of Electrospun Scaffolds for the Expansion of Precursors of Insulin-producing Cells." 2010, Presented at Biomedical Engineering Society Meeting,
  • "Validation and Characterization of an Acoustic Sensor Based on PVDF Micropillars and Patterned Electrodes." 2010, Presented at 3rd Annual Meeting of the ASME/AIAA Smart Materials, Adaptive Structures, and Intelligent Systems Conference,
  • "Polymer MEMS for Measuring Single Cell Forces." 2010, Presented at ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference,
  • "Zirconia Microtextured Surfaces Using Soft Lithography." 2010, Presented at 88th General Session and Exhibition of the International Association for Dental Research,
  • "Microstamped Silica Films on Curved Zirconia Surfaces." 2010, Presented at 88th General Session and Exhibition of the International Association for Dental Research,
  • "Anisotropic Microtextured Silica Thin Films on Zirconia." 2010, Presented at 88th General Session and Exhibition of the International Association for Dental Research,
  • "Portland Particulated Silica Coatings – Comparison between Two Coating Techniques." 2010, Presented at 88th General Session and Exhibition of the International Association for Dental Research,
  • "Micro-Structured Silica Coatings with HA Particles Using a PDMS Stamp." 2009, Presented at Materials Research Society Meeting,
  • "Combination of Micro- And Nanofabricated Structures for the Production of Biomimetic Tissue Engineering Scaffolds." 2009, Presented at Biomedical Engineering Society Meeting,
  • "Layer by Layer Microassembly of Multifunctional Bone Tissue Engineering Scaffolds." 2009, Presented at Biomedical Engineering Society Meeting,
  • "Polymer Microraceways for Characterization of Glioblastoma Cell Migratory Patterns." 2009, Presented at Biomedical Engineering Society Meeting,
  • "Controlled Pancreatic Cluster Formation on a Bed of Electrospun Fibers." 2009, Presented at Biomedical Engineering Society Meeting,
  • "Silica Coatings with Portland cement Particles for Dental Implant Surfaces Based on Sol-Gel Micromolding." 2009, Presented at Biomedical Engineering Society Meeting,
  • "Microfabricated SiO2 Thin Films on Structural Dental Ceramics." 2009, Presented at Bioceramics 22. International Symposium on Ceramics in Medicine,
  • "High throughput assembly of spatially controlled microscale tissue subunits." 2009, Presented at Society for Biomaterials Meeting,
  • "Bioactive Portland cement porous scaffolds." 2009, Presented at Society for Biomaterials Meeting,
  • "Design and microfabrication of a PVDF acoustic sensor." 2009, Presented at ASME Conference on Smart Materials, Automotive applications I,
  • "Acoustic sensor with PVDF micropillars and patterned electrodes." 2008, Presented at ASME Conference on Smart Materials, Adaptive Structures and Intelligent Systems,
  • "Polymer Micro-Nano Devices for Applications in Guided Cell Assembly." 2008, Presented at Biomedical Engineering Society Meeting,
  • "Spatially Controlled Aggregation of Pancreatic Cells in Microfabricated Wells on Polymer Membranes." 2008, Presented at Biomedical Engineering Society Meeting,
  • "Simultaneous Evaluation of Cell Proliferation and Gene Expression Via MTT-Coupled Real-Time RTPCR." 2008, Presented at Biomedical Engineering Society Meeting,
  • "Microwell Cell Traps for High Throughput Cell Isolation." 2008, Presented at . Biomedical Engineering Society Meeting,
  • "Microfabrication of Zeolite Membrane on Patterned Porous Alumina Support Using Soft Lithography." 2008, Presented at 12th International Meeting on Chemical Sensors,
  • "Evaluation of housekeeping gene stability during human bone marrow mesenchymal stem cells differentiation to the osteogenic lineage." 2008, Presented at 6th Annual Meeting of the International Society for Stem Cell Research,
  • "Biocompatibility, Bioactivity and Mechanical Properties of Portland cement and Portland cement-Metakaolin Blends for Bone Tissue Engineering Applications." 2008, Presented at Materials Research Society Meeting,
  • "Soft Lithographic Production of Electroactive Polyvinylidene Fluoride Microstructures for BioMEMS Technologies." 2008, Presented at BioMED 2008,
  • "Development of PDMS-reinforced Portland cement porous scaffolds for load-bearing bone tissue engineering applications." 2007, Presented at Second International Conference on Mechanics of Biomaterials and Tissues,
  • "Fabrication methods for polymer MEMS." 2007, Presented at American society for precision engineering,
  • "Portland cement – Metakaolin blends as novel biomaterials for load bearing bone tissue engineering." 2007, Presented at Biomedical Engineering Society Meeting,
  • "Microfabricated Membranes for Isolation and Morphological Control of Cells." 2007, Presented at Biomedical Engineering Society Meeting,
  • "Fabrication of piezoelectric polyvinylidene fluoride (PVDF) microstructures by soft lithography for tissue engineering and cell biology applications." 2007, Presented at Materials Research Society Meeting,
  • "Fabrication of Biocompatible Portland cement Porous Scaffolds for Bone Tissue Engineering Applications." 2006, Presented at The 8th New Jersey Symposium on Biomaterials Science,
  • "Bioactive Coatings on Portland cement Substrates: Surface precipitation of Apatite-like Crystals." 2006, Presented at Materials Science & Technology 2006 Conference,
  • "Microfabrication of Degradable Polymer Tissue Engineering Scaffolds." 2006, Presented at Materials Science & Technology 2006 Conference,
  • "Biocompatibility improvement of Portland cement through the introduction of pozzolanic materials: Effects of Metakaolin on the cytotoxicity." 2005, Presented at . 2nd Colombian Congress of Bioengineering and Biomedical Engineering,
  • "Fabrication of Portland cement porous scaffolds: Potential applications as load bearing bone substitutes." 2005, Presented at 2nd Colombian Congress of Bioengineering and Biomedical Engineering,
  • "Fabrication and Biocompatibility testing of Plaster of Paris Porous Scaffolds." 2005, Presented at 2nd Colombian Congress of Bioengineering and Biomedical Engineering,
  • "Bone Tissue Engineering applications of Portland cement Phase I: Biocompatibility studies – Effects of Calcium Hydroxide." 2005, Presented at Third International Materials Congress,
  • "Biocompatibility studies on Portland cement substrates: Potential Bone Tissue Engineering Applications." 2005, Presented at II Symposium about Biofactories, Biotechnology advances in Colombia,
  • "Microfabrication and in vitro evaluation of PLGA scaffolds." 2003, Presented at 1st Colombian Congress of Bioengineering and Biomedical Engineering,
  • "Identification of cardiac proteins in diabetic rats using proteomics." 2003, Presented at 1st Colombian Congress of Bioengineering and Biomedical Engineering,
  • "Identification of caspase substrates in carcinogenic cells using proteomics." 2003, Presented at 1st Colombian Congress of Bioengineering and Biomedical Engineering,

Papers in Proceedings

2016

  • Gygli, P.E.; Byers, S.; Higuita-Castro, N.; Czeisler, C. et al. "Deterministic transfection and genetic manipulation of organotypic brain slice cultures for ex vivo imaging." (4 2016).

2015

  • Bertani, P.; Chang, L.Q.; Gallego-Perez, D.; Malkoc, V. et al. "3D Si-based nanochannel platform for robust cell electroporation." (8 2015).

2014

  • Wang, L.J.; Li, L.; Ma, J.; Wu, Y. et al. "A microfluidic platform with continuous rapid cell trapping and micro/nano electroporation for single living cell study." (1 2014).

2012

  • Ma, J.; Kwak, K.J.; Wu, Y.; Gallego-Perez, D. et al. "Tethered immunolipoplex nanoparticle (tILN) device for detection of circulating tumor cells (CTCs) for lung cancer diagnosis." (12 2012).

2011

  • Pelaez-Vargas, A.; Gallego-Perez, D.; Fernandes, M.H.; Hansford, D. et al. "Microstructured coatings to study the behavior of osteoblast-like cells on hard materials." (5 2011).
  • Carvalho, A.; Pelaez-Vargas, A.; Gallego-Perez, D.; Fernandes, M.H. et al. "Adhesion and proliferation of mesenchymal stem cells on micropatterned thin films modified with nanohydroxyapatite particles." (5 2011).

2010

  • Xu, J.; Dapino, M.J.; Gallego-Perez, D.; Hansford, D. "Validation and characterization of an acoustic sensor based on PVDF micropillars and patterned electrodes." (12 2010).
  • Ferrell,Nicholas; Woodard,James; Gallego-Perez,Daniel; Higuita-Castro,Natalia; Hansford,Derek "POLYMER MEMS FOR MEASURING SINGLE CELL FORCES." (1 2010).

2009

  • Xu, J.; Dapino, M.J.; Perez, D.G.; Hansford, D. "Design and microfabrication of a pvdf acoustic sensor." (6 2009).

2008

  • Gallego, D.; Higuita, N.; Garcia, F.; Posada, O.M. et al. "Biocompatibility, bioactivity and mechanical properties of Portland cement and Portland cement-Metakaolin blends for bone tissue engineering applications." (12 2008).
  • Gallego, D.; Ferrell, N.; Higuita, N.; Hansford, D.J. "Soft lithographic production of electroactive polyvinylidene fluoride microstructures for BioMEMS technologies." (12 2008).

2007

  • D Gallego-Perez, N Ferrell, DJ Hansford "Fabrication of piezoelectric Polyvinylidene Fluoride (PVDF) microstructures by soft lithography for tissue engineering and cell biology applications." in MRS Spring Meeting 2007. (4 2007).

2006

  • D Gallego-Perez, N Higuita-Castro, F Garcia, N Ferrell, DJ Hansford "Bioactive coatings on Portland cement: Surface precipitation of apatite-like crystals." in Symposium on Advanced Processing of Biomaterials. (10 2006).

Patents

  • "Electroosmotic devices for fluid handling."
  • "Nano-engineered therapeutic stealth cells."
  • "Designer Extracellular Vesicles for Targeted Therapies Against Myeloid-Derived Suppressor Cells."
  • "Novel custom-made nanocarriers for lung inflammation therapy."
  • "Compositions and methods for reprogramming skin into insulin-producing tissue."
  • "Interpenetrating nanochannels for deep-topical tissue nanoelectroinjection."
  • "Compositions and methods for reprogramming somatic cells into induced endothelial cells."