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Jennifer Leight

  • Assistant Professor, Biomedical Engineering
  • 291 Bevis Hall
    1080 Carmack Road
    Columbus, OH 43210
  • 614-685-9147

About

Education

  • B.S., Tulane University, 2004
  • Ph.D., University of Pennsylvania, 2011

 

Key Honors and Distinctions

  • US Frontiers of Engineering Symposium Participant, National Academy of Engineering (2016)
  • Chair, Graduate Research Seminar – Signal Transduction by Engineered ECM Gordon Conference (2010)
  • National Science Foundation Graduate Research Fellowship (2005)

 

RESEARCH AREAS – Leight Lab for Engineering the Cancer Microenvironment [leightlab.engineering.osu.edu]

  • Polymers; Biomaterials; Molecular, cell and tissue engineering; Cancer

  • PUBLICATIONS

leightlab.engineering.osu.edu/publications

The Leight Lab focuses on the development and utilization of new tools to understand how the microenvironment is remodeled during tumorigenesis and how this remodeling impacts cancer cell function and response to treatment. Specifically, we use fluorogenic peptide biosensors in tunable 3D hydrogel platforms to investigate microenvironmental regulation of protease activity, cell viability, and migration. Applications for our technology are far reaching, not only for screening cancer therapeutics, but also in other contexts in which protease activity plays an important role, such as heart disease, arthritis, and tissue regeneration.

Honors

  • January, 2018

    Herman R. Weed Excellence in Teaching Award.

  • January, 2016

    US Frontiers of Engineering Symposium Participant.

  • January, 2005-
    January, 2008

    Graduate Research Fellowship.

  • January, 2000-
    January, 2004

    Distinguished Scholar Award.

Chapters

2007

  • Johnson, K.R.; Leight, J.L.; Weaver, V.M.. 2007. "Demystifying the Effects of a Three-Dimensional Microenvironment in Tissue Morphogenesis."

Journal Articles

2019

  • Casadei, L.; Calore, F.; Braggio, D.A.; Zewdu, A. et al., 2019, "MDM2 derived from dedifferentiated liposarcoma extracellular vesicles induces MMP2 production from preadipocytes.." Cancer Res
  • Campbell, W.A.; Deshmukh, A.; Blum, S.; Todd, L. et al., 2019, "Matrix-metalloproteinase expression and gelatinase activity in the avian retina and their influence on Müller glia proliferation.." Exp Neurol 320, 112984 - 112984.
  • Fakhouri, A.S.; Weist, J.L.; Tomusko, A.R.; Leight, J.L., 2019, "High-Throughput Three-Dimensional Hydrogel Cell Encapsulation Assay for Measuring Matrix Metalloproteinase Activity.." Assay and drug development technologies 17, no. 3, 100-115 - 100-115.
  • Fakhouri, A.S.; Leight, J.; Weist, J.L., 2019, "High-Throughput Three-Dimensional Hydrogel Cell Encapsulation Assay for Measuring Matrix Metalloproteinase Activity.." Assay and Drug Development Technologies
  • Fakhouri, A.S.; Leight, J.L., 2019, "Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels." JOVE-JOURNAL OF VISUALIZED EXPERIMENTS no. 143,
  • Deshmukh, A.A.; Weist, J.L.; Leight, J.L., 2019, "Detection of Protease Activity by Fluorescent Peptide Zymography." JOVE-JOURNAL OF VISUALIZED EXPERIMENTS no. 143,
  • Jones, C.E.; Hammer, A.M.; Cho, Y.J.; Sizemore, G.M. et al., 2019, "Stromal PTEN Regulates Extracellular Matrix Organization in the Mammary Gland." Neoplasia (United States) 21, no. 1, 132-145 - 132-145.

2018

  • Shin, D.S.; Tokuda, E.Y.; Leight, J.L.; Miksch, C.E. et al., 2018, "Synthesis of Microgel Sensors for Spatial and Temporal Monitoring of Protease Activity." ACS Biomaterials Science and Engineering 4, no. 2, 378-387 - 378-387.
  • Deshmukh, A.A.; Weist, J.L.; Leight, J.L., 2018, "Detection of proteolytic activity by covalent tethering of fluorogenic substrates in zymogram gels." BIOTECHNIQUES 64, no. 5, 203-210 - 203-210.
  • Barnhouse, V.R.; Weist, J.L.; Shukla, V.C.; Ghadiali, S.N. et al., 2018, "Myoferlin regulates epithelial cancer cell plasticity and migration through autocrine TGF-β1 signaling." Oncotarget 9, no. 27, 19209-19222 - 19209-19222.

2017

  • Leight, J.L.; Drain, A.P.; Weaver, V.M., 2017, "Extracellular Matrix Remodeling and Stiffening Modulate Tumor Phenotype and Treatment Response." Annual Review of Cancer Biology 1, no. 1, 313-334 - 313-334.
  • Shukla, V.; Barnhouse, V.; Ackerman, W.E.; Summerfield, T.L. et al., 2017, "Cellular Mechanics of Primary Human Cervical Fibroblasts: Influence of Progesterone and a Pro-inflammatory Cytokine." Annals of Biomedical Engineering 1-11 - 1-11.

2015

  • Sridhar, B.V.; Brock, J.L.; Silver, J.S.; Leight, J.L. et al., 2015, "Development of a cellularly degradable PEG hydrogel to promote articular cartilage extracellular matrix deposition." Advanced Healthcare Materials 4, no. 5, 702-713 - 702-713.
  • Leight, J.L.; Tokuda, E.Y.; Jones, C.E.; Lin, A.J. et al., 2015, "Multifunctional bioscaffolds for 3D culture of melanoma cells reveal increased MMP activity and migration with BRAF kinase inhibition." Proceedings of the National Academy of Sciences of the United States of America 112, no. 17, 5366-5371 - 5366-5371.

2014

  • Tokuda, E.Y.; Leight, J.L.; Anseth, K.S., 2014, "Modulation of matrix elasticity with PEG hydrogels to study melanoma drug responsiveness." Biomaterials 35, no. 14, 4310-4318 - 4310-4318.

2013

  • Leight, J.L.; Alge, D.L.; Maier, A.J.; Anseth, K.S., 2013, "Direct measurement of matrix metalloproteinase activity in 3D cellular microenvironments using a fluorogenic peptide substrate.." Biomaterials 34, no. 30, 7344-7352 - 7344-7352.

2012

  • Leight, J.L.; Wozniak, M.A.; Chen, S.; Lynch, M.L. et al., 2012, "Matrix rigidity regulates a switch between TGF-beta 1-induced apoptosis and epithelial-mesenchymal transition." MOLECULAR BIOLOGY OF THE CELL 23, no. 5, 781-791 - 781-791.
  • Leight, J.L.; Liu, W.F.; Chaturvedi, R.R.; Chen, S. et al., 2012, "Manipulation of 3D cluster size and geometry by release from 2D micropatterns." Cellular and Molecular Bioengineering 5, no. 3, 299-306 - 299-306.
  • 2012, "Matrix rigidity regulates a switch between TGF-beta 1-induced apoptosis and epithelial-mesenchymal transition." MOLECULAR BIOLOGY OF THE CELL 23, no. 5,

2009

  • Gehler, S.; Baldassarre, M.; Lad, Y.; Leight, J.L. et al., 2009, "Filamin A-β1 integrin complex tunes epithelial cell response to matrix tension." Molecular Biology of the Cell 20, no. 14, 3224-3238 - 3224-3238.

2007

  • Janmey, P.A.; McCormick, M.E.; Rammensee, S.; Leight, J.L. et al., 2007, "Negative normal stress in semiflexible biopolymer gels.." Nat Mater 6, no. 1, 48-51 - 48-51.

Unknown

  • "Multifunctional bioscaffolds for 3D culture of melanoma cells reveal increased matrix metalloproteinase activity and migration in response to BRAF/MEK inhibitors.." PNAS

Presentations

  • "Hydrogel culture platforms to investigate the regulation of cancer cell function.." 2014, Presented at Ohio State University,,
  • "Multiplex biomaterials for high throughput cancer drug screening.." 2015, Presented at Big Ten Speaker Exchange,
  • "3D hydrogel culture platforms to direct and measure cancer cell function.." 2014, Presented at Signaling and Cellular Regulation Symposium,
  • "Cellular remodeling of the tumor microenvironment." 2017, Presented at Seminar Speaker, Department of Materials Engineering,
  • "In vitro models to study cellular remodeling of the tumor microenvironment." 2019, Presented at Department of Chemical and Biomolecular Engineering,

Papers in Proceedings

2018

  • Deshmukh, A.A.; Weist, J.L.; Leight, J. "In-Gel Fluorescent Peptide Zymography for the Analysis of Cell Secreted Proteases." in Society for Biomaterials Annual Conference. (1 2018).
  • Deshmukh, A.A.; Weist, J.L.; Leight, J. "Engineering Degradable Microenvironments." in Gordon Research Conference Signal Transduction by Engineered ECMs. (1 2018).
  • Fakhouri, A.; Weist, J.L.; Tomusko, A.; Yee, L.D. et al. "Development of a High Throughput 3D Biomaterials Platform to Measure Protease Activity in Breast Cancer Patients." in Society for Biomaterials Annual Meeting. (1 2018).
  • Jones, C.E.; Hammer, A.; Cho, Y.; Sizemore, G. et al. "Stromal PTEN Regulates Extracellular Matrix Organization in the Mammary Gland." in The American Society for Matrix Biology Biannual Meeting. (1 2018).

2017

  • Jones, C.E.; Cho, Y.; Cukierman, E.; Hammer, A. et al. "Changes in Extracellular Matrix Structure Due to Fibroblast Genotype Direct Cancer Cell Behavior." in Biomedical Engineering Society Annual Meeting. (1 2017).
  • Hay, B.R.; Reader, B.; Christman, J.W.; Leight, J. et al. "The Role Of Tlr Signaling In Regulating Collagen Uptake And Degradation In Macrophages Following Bleomycin-Induced Pulmonary Fibrosis." in International Conference of the American-Thoracic-Society (ATS). (1 2017).
  • Weist, J.; Barnhouse, V.; Shukla, V.; Ghadiali, S.N. et al. "Myoferlin depletion reduces autocrine TGF-beta 1 production to regulate epithelial-mesenchymal transition in breast cancer cells.." in Annual Joint Meeting of the American-Society-for-Cell-Biology and the European-Molecular-Biology-Organization (ASCB/EMBO). (1 2017).
  • Kaltenmark, K.J.; Jones, C.E.; Weist, J.L.; Leight, J.L. "The role of matrix stiffness in the regulation of matrix metalloproteinase activity." in National Collegiate Research Conference. (1 2017).

2016

  • Barnhouse, V.; Weist, J.L.; Leight, J. "Myoferlin depletion in MDA-MB-231 breast cancer cells reduces autocrine TGF-beta1 production." in Biomedical Engineering Society Annual Meeting. (1 2016).

2015

  • Tokuda, E.Y.; Leight, J.; Anseth, K.S. "Multifunctional hydrogel assays for 3D culture of melanoma cells reveal increased matrix metalloproteinase activity and migration in response to BRAF/MEK inhibitors." in American Chemical Society. (1 2015).
  • Leight, J.; Tokuda, E.Y.; Anseth, K.S. "Multifunctional bioscaffolds for 3D culture of melanoma cells reveal increased MMP activity and migration with BRAF kinase inhibition." in Gordon Research Conference Matrix Metalloproteinases. (1 2015).
  • Sridhar, B.; Brock, J.; Silver, J.; Randolph, M. et al. "Development of a cellularly degradable PEG hydrogel to promote articular cartilage extracellular matrix deposition." in American Chemical Society. (1 2015).
  • Leight, J.; Tokuda, E.; Anseth, K. "Functionalization of hydrogels with matrix metalloproteinase-sensitive fluorogenic biosensors to measure cancer cell response to drug treatment." (3 2015).

2014

  • Tokuda, E.Y.; Leight, J.; Anseth, K.S. "Matrix elasticity regulates melanoma cell survival." in Society for Biomaterials. (1 2014).
  • Leight, J.L.; Tokuda, E.Y.; Anseth, K.S. "Measuring protease activity in 3D microenvironments." in American Society for Matrix Biology. (1 2014).
  • Leight, J.L.; Tokuda, E.Y.; Anseth, K.S. "Functionalization of hydrogels with a MMP-sensitive fluorogenic substrate to measure cellular response to drug treatment.." in Society for Biomaterials. (1 2014).

2013

  • Tokuda, E.Y.; Leight, J.L.; Anseth, K.S. "Elucidating the role of microenvironmental cues on melanoma drug resistance." in Society for Biomaterials. (1 2013).
  • Leight, J.; Alge, D.A.; Maier, A.J.; Anseth, K.S. "Direct measurement of MMP activity in 3D cellular microenvironments using fluorigenic peptide substrates.." in Biomedical Engineering Society Annual Meeting. (1 2013).

2012

  • Alge, D.A.; Leight, J.L.; Anseth, K.S. "Tissue engineering implications of inflammatory cytokine induced MMP upregulation by hMSCs.." in Biomedical Engineering Society Annual Meeting. (1 2012).
  • Leight, J.L.; Alge, D.A.; Anseth, K.S. "Characterizing MMP expression using modular fluorescent peptide biosensors.." in Polymer Networks Group Annual Conference. (1 2012).

2010

  • Leight, J.; Chen, C.S. "Mechanical properties of the microenvironment regulate TGFbeta-inducedcell fate through AKt." in Gordon Research Conference Signal Transduction by Engineered ECMs. (1 2010).
  • Leight, J.L.; Wozniak, M.A.; Chen, S.; Lynch, M.L. et al. "Matrix compliance regulates a switch between TGF-β1-induced apoptosis and epithelial-mesenchymal transition.." in American Society for Cell Biology Annual Meeting. (1 2010).

2009

  • Leight, J.L.; Chen, C.S. "Mechanical properties of the microenvironment regulate TGF-β1-induced cell fate through Akt.." in American Society for Cell Biology. (1 2009).
  • Leight, J.L.; Chen, C.S. "Mechanical properties of the microenvironment regulate TGF-β1-induced cell fate through Akt.." in American Society for Cell Biology Annual Meeting. (1 2009).

2008

  • Leight, J.L.; Chen, C.S. "Mechanical properties of the microenvironment modulate the oncogenic potential of TGF-β.." in Signaling by Adhesion Receptors Gordon Conference. (1 2008).
  • Leight, J.L.; Chen, C.S. "Mechanical properties of the microenvironment modulate the oncogenic potential of TGF-β.." in Signal Transduction by Engineered Extracellular Matrices. (1 2008).

2007

  • Leight, J.L.; Chen, C.S. "Microenvironmental cues regulate epithelial-mesenchymal transition." in Biomedical Engineering Society Annual Meeting,. (1 2007).

2005

  • Leach, J.B.; Leight, J.L.; Johnson, K.R.; Zahir, N. et al. "Engineered 3D models to study force-dependent mammary morphogenesis and malignancy.." in American Society for Cell Biology Annual Meeting. (1 2005).
  • Leight, J.L.; Zahir, N.; Lakins, J.; Weaver, V.M. "Force-dependent apoptosis resistance and breast tumorigenesis." in American Society for Cell Biology Annual Meeting. (1 2005).
  • Zahir, N.; Leight, J.L.; Alston-Mills, B.; Weaver, V.M. "Matrix stiffness differentially regulates Jun Kinase to modulate apoptosis responsiveness of a mammary epithelium." in American Society for Cell Biology Annual Meeting. (1 2005).
  • Leight, J.L.; Chatterjee, C.; Zahir, N.; Nuth, M. et al. "Spatial-mechanical regulation of apoptosis resistance in breast cancer." in Biomedical Engineering Society Annual Meeting. (1 2005).